He studied at the Saint St. Petersburg Theological Academy, graduating in 1878.
From 1879-1881 he went to America as a clergyman at the church attached to the Russian embassy in New New York Returning to Saint St. Petersburg, he was professor of comparative theology from 1883–1885, and then of ancient history from 1885-1904. His main work, the Lopukhin Bible, a three-volume Bible with commentary, was published in 1904.
He is buried at the Nikolskoe Cemetery of Alexander Nevsky Lavra.