Alexander Stepanovich Afanasyev-Chuzhbinsky graduated from the Nezhin Lyceum in 1835.
His first Ukrainian poem came out in 1841. His Ukrainian poems were collected in From My Heart and published in 1855. Afanasyev-Chuzhbinsky's two-volume ethnographic work, A Journey to the Southern Russia (Saint Petersburg, 1861) came as a result of his 1856 journey to Pridneprovye (Dnieper River region).
Alexander Stepanovich took part in compiling the Dictionary of the Ukrainian Language which was endorsed by the Russian Academy of Sciences, even if criticized by several Ukrainian language scholars.
Alexander Stepanovich launched Peterburgsky Listok (Petersburg Leaflet) in 1867, and later, in the 1870s Magazin Inostrannoi Literatury (Magazine of Foreign Literature) which he was also the editor of. He translated the works by James Fenimore Cooper, Henryk Rzewuski, Józef Ignacy Kraszewski and Józef Korzeniowski. In 1851 he compiled and published the Gallery of Polish Writers in 5 volumes.
Alexander Stepanovich died in Saint Petersburg, aged 59.