On the occasion of this marriage he had the Urach Palace in Bad Urach renovated: the Golden Hall was constructed, which is today one of the most beautiful Renaissance ballrooms in Germany. They apparently had a very happy marriage. Her husband died in 1628.
In 1630, she began an extensive renovation of Brackenheim Castle, which was promised to her as her wittum.
The castle contained an art room with 155 paintings, at the time the second largest collection in Württemberg. The art room was well preserved until her death, despite the Thirty Years" War ravaging the country around lieutenant
During the renovation of her castle, she lived at Schloss Kirchheim Castle in Kirchheim unter Teck and in Stuttgart. She never lived in Brackenheim, despite the Castle, the city and the district forming her wittum.
Nevertheless, she was considered a benefactor of the city, due to her dedication to the city during the Thirty Years" War and to the foundations she founded.
After Frederick Louis died on 26 January 1631, the regency was taken up by Sophie Barbara and Julius Frederick, Duke of Württemberg-Weiltingen, with Sophie Barbara being "high regent". She therefore moved back to Stuttgart in 1632 and became politically active. After the Battle of Lutzen in late 1632, Julius Frederick joined the war on the Swedish side.
His goal was to drive enemy troops out of the country, as well as the Catholic former owners of secularized church properties.
Despite his successes, he was accused of acting selfishly. The councillors and the Estates managed to drive him out of the regency.
After Emperor Ferdinand II declared Eberhard III to be an adult, he took up government on 8 May 1633. Württemberg was then looted and pillaged.
Duke Eberhard and his entire court hastily fled into exile in Strasbourg.
Barbara Sophia died in Strasbourg in 1636 and was buried in the Stiftskirche, Stuttgart. Henrietta (1610–1623)
Eberhard III (1614–1674), Duke of Württemberg, married
in 1637 Wild-Rhinegravine Anna Catherine of Salm-Kyrburg (1614–1655)
in 1656 Countess Maria Dorothea of Oettingen (1639–1698)
Frederick (1615–1682), Duke of Württemberg-Neuenstadt
married in 1653 Princess Clara Augusta of Brunswick-Wolfenbüttel (1632–1700)
Ulrich (1617–1671), Duke of Württemberg-Neuenbürg, married
in 1647 Countess Sophia Dorothea of Solms-Sonnewalde (1622–1648)
in 1651 Princess Isabella d"Arenberg (1623–1678)
Anna Johanna (1619–1679)
married in 1647 Duke Leopold Frederick of Württemberg-Montbéliard (1624–1662).