From there she moved with her family to Beauty Point, attending SCECGS Redlands for six months until enrolling at Brent Street Performing Arts High School where she studied all aspects of performing arts
Best made her television debut in 2007 in Home and Away, starring as Annie Campbell until 2010. Three months into a full-time Global Studies course at the University of Technology, Sydney, Best accepted the role of Cheryl Haynes in Puberty Blues. From a young age, Best has appeared regularly as a fashion model in such magazines as Barbie, Total Girl, Oyster and Cosmopolitan.
She was the face of Mission Metallicus Clothing brand and appeared on Comedy Incorporated, and has modelled for Supre.
Best has been an ambassador for the charities CURE OUR KIDS and WORLD VISION. Charlotte is best known for her role as Annie Campbell in the long running television series Home and Away between 2007 and 2010. Her character went on a student exchange to Japan, commencing October 2009, which was originally scheduled to be for 6 months.
She returned on screen in March 2010, on a 6-week holiday break from Japan, before leaving again. In March 2012, it was announced, that Best had been cast as Cheryl Hayes in Network Ten"s drama series Puberty Blues, which is based on the 1979 novel of the same name by Gabrielle Carey and Kathy Lette.