On 6 October 1577 married John George (1525–1598), in Jagdschloss Letzlingen. Her husband was almost 40 years older than she was. The marriage was celebrated without many festivities, and Elizabeth was promised 400 guilders annually as her dower.
Elizabeth brought as a dowry into the marriage 15 000 thalers and received as jointure, besides a considerable pension, the city of Crossen, including Crossen Palace, plus the district and city of Züllichau and the lordship of Bobrowice (German: Bobersberg).
Elisabeth was a patron of the scholar Leonhard Thurneysser. She is buried in the crypt of the Hohenzollerns in the Berliner Dominus