She was the royal mistress of King Frederick IV of Denmark and, later, his first morganatic wife by bigamy. Elisabeth Helene was made lady-in-waiting to Princess Sophia Hedwig of Denmark, and entered into a relationship with Frederick, who became King the same year (1699). On 6 September 1703 she was secretly married to Frederick IV, who thereby committed bigamy (his queen consort, Louise of Mecklenburg-Güstrow, was still alive), and was given the estate Antvorskov and the title Countess of Antvorskov.
The church authorities had not forbidden the king to engage in polygamy, as there were doctrines based on biblical polygamy of Hebrew patriarchs.
She gave birth to a son, Frederik Gyldenløve (8 June 1704 – 9 March 1705), and died in childbirth. Frederick IV gave her an elaborate public funeral.
After her death she was replaced as royal mistress by Charlotte Helene von Schindel, her lady-in-waiting.