Eugène graduated from the University of Pennsylvania in 1861 with a bachelor of arts degree. After he graduated, he was an assistant to Lammot du Pont at the Brandywine Mills laboratory and by 1886 filed two patent applications (gunpowder press and new variety of powder, brown prismatic).
He became junior partner in 1864. As senior partner, du Pont saw the completion of a new office in Wilmington and the new invention called the telephone. He also saw the rise of the dynamite industry and helped form the Eastern Dynamite Company in 1895.
1912, the Eastern Dynamite Company formally merged with DuPont.