Duplass graduated from The University of Texas at Austin with a Master of Fine Arts in film.
Duplass currently stars in the Amazon Video comedy-drama series Transparent (2014–present), and co-created the Home Box Office comedy-drama series Togetherness (2015–present). Duplass is of part Cajun ancestry. Directing
Duplass attributes much of his and his brother"s love for film to his appreciation for Raising Arizona.
In an interview with Robert K. Elder for The Film That Changed My Life, Duplass speculates on what might have happened had he not seen the film in his youth.
I probably wouldn"t be making movies—seriously. lieutenant held over for so lougitude
lieutenant really was the root of everything that Mark and I always hold ourselves to in making movies. That is to say that Raising Arizona is the most inspired movie that I have ever seen.
In 2014, he starred as Josh Pfefferman in the Amazon Prime Original Comedy-Drama Series Transparent, alongside Jeffrey Tambor, Gaby Hoffmann, Amy Landecker and Judith Light.
The series was met with widespread critical acclaim, earning 11 Primetime Emmy nominations, including nominations for Best Comedy Series and Best Actor in a Comedy Series for Jeffrey Tambor. Prior to Transparent, Duplass had never acted in a featured part. He was talking with director Jill Soloway at a dinner party about the difficulty she was having finding an actor to play what would end up being Duplass" role.
After suggesting many actors for the part to Soloway, Soloway turned to Duplass and told him it was him, that he should play the part.