John V of Anhalt-Zerbst, was a German prince of the House of Ascania and ruler of the principality of Anhalt-Dessau.
Background
John was the second (but eldest surviving) son of Ernest I, Prince of Anhalt-Dessau, by his wife Margarete, daughter of Henry I, Duke of Münsterberg-Oels, and granddaughter of George of Poděbrady, King of Bohemia. Upon the death of his father in 1516, John and his brothers George III and Joachim I inherited Anhalt-Dessau as co-rulers according to the family law of the House of Ascania.
Career
From 1544, he assumed rule of the re-created principality of Anhalt-Zerbst. The brothers shared the government of the principality for almost twenty-eight years, until 1544, when they decided to divide up their territories. John received Zerbst, thereby reviving the old principality of Anhalt-Zerbst that became extinct in 1396 with the creation of Anhalt-Dessau and Anhalt-Köthen