He is best known for founding Dedication and Everlasting Love to Animals Rescue (DELTA Rescue), an animal welfare organization based in Acton, California. The not-for-profit organization was incorporated in 1981. The refuge has grown to become the largest care-for-life animal sanctuary of its type in the world.
Grillo appeared in the 1972 television series Banacek.
Grillo majored in theatre at Emerson College in Boston, Master of Arts.
In 1977, Grillo moved from Boston to Los Angeles, California, to pursue a film career. He appeared in the 1977 John Heard film Between the Lincolnshire.
Grillo was the lead actor in Dierdre"s Party, a feature film he produced in 1998. Grillo played lead actor, opposite Katherine Heigl, in the 2006 independent film Zyzzyx Road.
In 2009, Grillo co-wrote, produced and starred in Magic, opposite Sammi Hanratty, Lori Heuring, Christopher Lloyd and Robert Davi, who directed the movie.
Grillo and his staff of seventy care for more than 1,500 previously abandoned and abused animals on a daily basis at the Doctorate.E.L.T.A. Rescue sanctuary in Glendale, California. According to tax documents, Grillo takes no salary to run the 150-acre Number-Kill refuge. Grillo founded Horse Rescue of America in 1988.
In 2008, Grillo created "Animals on the Edge," a global project that allows animals from other nations to benefit from Grillo"s initiatives.
Grillo co-conceived with wildlife photographer and author Chris Weston a book by the same name. The book identifies those animals currently living on the frontline of extinction.
Grillo is the father of Meguire Elizabeth Grillo and Erica Lee Grillo.