Livia was the daughter of Ippolito della Rovere (illegitimate son of Cardinal Giulio Feltrio della Rovere) and Isabella Vitelli dei Signori dell"Amatrice. Livia"s second cousin, Francesco Maria II della Rovere (Duke of Urbino) was married to Donna Lucrezia d"Este, the daughter of Ercole II d"Este, Duke of Ferrara.
Ippolito was later legitimised and named Marchese di San Lorenzo. was married to Claudia de" Medici in 1620 and they had one child, Vittoria della Rovere. When died suddenly on 29 June 1623, after an epileptic attack, the Duchy was again without a male heir. Francesco again took up the title of Duke of Urbino but realised his situation was hopeless.
He forfeited his Duchy to Pope Urban VIII in 1625.
Francesco died in 1631. As the only heir, male or female, Vittoria della Rovere inherited what was left in property, art and funds of the Duchy.
Livia lived a further ten years after her husband"s death. She lived primarily as a recluse, leaving Castelleone di Suasa only for significant events.
She died 6 July 1641.
