Around 1900, she moved with her family to Montreal, where she studied music with Mission Lemire and Béatrice Lapalme.
She came from a musical family, being the sister of Pierre-Aurèle Asselin and the great aunt of André Asselin. Asselin made her singing debut on April 25, 1919 in the role of Jeanne in Louisiana Basoche by André Messager, which was performed in the Théâtre Français in Montreal. In 1920, Asselin opened a vocal studio on Saint-Denis Street in Montreal.
That year she performed many concerts, featuring performances with Émile Gour, Germain Lefevbre, Hercule Lavoie and Blanche Gonthier.
A series of radio performances with José Delaquerrière, Jeanne Maubourg, Blanche Archambault, Germaine Lebel, André Durieux and Maurice Jacquet broadcast on CKAC were also heard in New England, and as a result the group was invited to perform a concert broadcast on a new station in Springfield, Illinois. She remained a vocal teacher in Montreal for her life, moving studios to Rue Saint Hubert in the 1930s and Saint-Joseph Boulevard East in the 1950s.
She died in Montreal in 1971.