When she was sixteen, she began working as a servant in Budapest. She went on to complete her education in Prague, Paris and Vienna. In 1907, she published a collection of stories Povídky z pekla a jiné (Stories from Hell and other stories) and a novel Panenství (Maidenhood).
Her writing concerns itself with the oppression of the working class and of women.
She also wrote literature for children. Majerová was married twice: first to the journalist Josef Stivín and then to the graphic artist Slavoboj Tusar.
She died in Prague at the age of 84. The 1937 film Virginity, directed by Otakar Vávra, was based on her novel Panenství.
Her novel Siréna was the basis for the screenplay for the 1947 film of the same name with English title The Strike, directed by Karel Steklý.
The 1947 film received a Golden Lion at the Venice Film Festival. Czechoslovakian-Canadian author Josef Škvorecký has said that his character Marie Burdychova in The MIracle Game was physically based on Marie Majerová.
Communist Party of Czechoslovakia, Czechoslovakian Social Democratic Party.
She was a member of the Czechoslovak Communist Party from its inception and was also involved in the feminist movement.