Born and raised at Possenhofen Castle, Mathilde was a younger sister of (among others) Duke Karl-Theodor in Bavaria, Duchess Elisabeth in Bavaria and Duchess Marie Sophie in Bavaria. She was an older sister of (among others) Duchess Sophie in Bavaria. marqués de Peñaflor. However, the Two Sicilies were conquered by the Expedition of the Thousand under Giuseppe Garibaldi in 1861.
Garibaldi served the Kingdom of Sardinia which was in the process of Italian unification.
Lodovico was still the heir of Francis as head of a deposed Royal House. He retained this position for the rest of his life but predeceased Francis on 8 June 1886.
Titles and styles 30 September 1843 – 5 June 1861: Her Royal Highness Duchess Mathilde Ludovika in Bavaria 5 June 1861 – 8 June 1886: Her Royal Highness The Countess of Trani, Princess of Bourbon-Two Sicilies, Duchess in Bavaria 8 June 1886 – 18 June 1925: Her Royal Highness The Dowager Countess of Trani, Princess of Bourbon-Two Sicilies, Duchess in Bavaria.