Career
He primarily painted religious-themed works for church commissions. Tzafouris is known from documents to have been active in Crete. Five of his signed icons survive, which were executed in the maniera italiana, as practiced by Cretan painters for western commissions.
One work executed by Nikolaos Tzafouris was a triptych Pietà with Saints Francis and Mary Magdalen which can be found at the Ashmolean Museum.