Born in Rome, Paola Senatore was as an actress who worked in Italian genre films during the 1970s, including poliziotteschi, commedia sexy all"italiana and giallo films. These included cult films such as Women in Cell Block 7 (1973), Ricco the Mean Machine (1973), The Flower with the Petals of Steel (1973), The Killer Reserved Nine Seats (1974), Salon Kitty (1976), Like Rabid Dogs (1976), Oil! (1977), Emanuelle in America (1977), L"infermiera di notte (1979), Where Can You Go Without the Little Vice? (1979), Images in a Convent (1979), Action (1980), Umberto Lenzi"s cannibal film Eaten Alive! (1980), and Louisiana dottoressa preferisce i marinai (1981). In the mid 1980s, after two soft-core erotic films directed by Joe Doctorate"Amato and because of drug problems and pregnancy, she accepted starring in pornographic magazines and in a hard-core film, Non stop.. sempre buio in sala.
Her film career ended in September 1985 when she was arrested for possession and trafficking of drugs.