In 1805, he went to Rome where he enrolled at the Accademia di San Luca and studied with Antonio Canova.
His first teacher was an Italian sculptor and woodcarver who was living on Corfu. After returning to Corfu, he participated in creating an "Academy of Sciences", sponsored by the French government. In 1811, he opened a private art school.
The first such modern school to be established in Greece.
When Corfu became a protectorate of the British Empire in 1815, Lord High Commissioner Thomas Maitland transformed the school into the "Public Academy of Fine Arts". By 1819, it had eighty students.
The painter Dionysios Vegias was one of his pupils. In 1824, when Lord Guilford created the Ionian Academy, Prosalentis was one of the first to be offered a teaching position there.
He accepted, but declined to take a salary, suggesting that the money be spent on making copies of the Elgin Marbles and other works that had been removed from the Parthenon, and using them for educational or restorative work.
He eventually received numerous free copies, creating scholarships from the money saved. Although not a wealthy man, he often gave private lessons for only the cost of materials and transportation. Eventually, casting and other foundry work damaged his health and he died in 1837, at the age of fifty-three.
In addition to his own sculpting, he also made sketches for works by other sculptors and pedestal panels for several monuments and busts.
He also did some painting, mostly of a religious nature.