He is perhaps best known for playing Corporal Charles "Chuck" Boyle on television"s Gomer Pyle, United States.M.C. during seasons two, three, and four from 1965 to 1968. Born in The Bronx, New York, Stuart launched his career performing in nightclubs and theatre. His stage credits include the Broadway musicals Beg, Borrow or Steal (1960) and Cafe Crown (1964).
He also appeared in numerous television commercials.
Roy Stuart died at age 78 of cancer at the Motion Picture Hospital, Woodland Hills, California. He is interred in Eden Memorial Park Cemetery, Mission Hills.