Thomas Chamberlayne was the son of Sir Thomas Chamberlayne, 1st Baronet and probably inherited the baronetcy on the death of his father on 6 October 1643, but the title (one conferred after 4 January 1642) being void under the Acting of Parliament (4 February 1652) then in force, on 6 October 1657 he accepted another baronetcy from the Lord Protector Oliver Cromwell, to whose Attorney General, Edmund Prideaux he was son-in-law.
This creation became, invalid after the Restoration, while his previous one was reinstated. He died late September or early November 1682. The baronetcy passed to James Chamberlayne, 3rd Baronet.
They had two daughters and coheirs:
Katharine, who inherited the estate of Wickham, became Viscountess Wenman, and, subsequently Countess of Abingdon.
Penelope, who inherited the estate of Northbrooke, married Robert Dashwood, who was created a baronet, in 1684, as "baronet of Northbrook".
