She speaks Italian, English, French and Spanish. Serafini has appeared in 29 films and television series since the early 2000s (decade), including: Il Divo (film), Colpo d"occhio, Il bene e il male, Il peccato e la vergogna and Valzer, which was screened at the 64th Venice International Film Festival. Serafini made her film début in the 2006 film Sono tornato al nord.
Serafini"s first significant role came in Roberto Cuzzillo"s Senza fine (2009) as a young homosexual - winning her critical and commercial recognition, as well as the Best Actress in a Leading Role Award at 2009 Chieti Film Festival directed by Roberto Faenza.
In 2011 Serafini was selected for four lead roles. Serafini, who has often been photographed by paparazzi with American actor and director Sean Penn, always refuses to comment about her private life.