Colfe was the fourth son of Amandus, Almantius, or Aymon Colfe and his wife, Catherine Bradfield, and uncle of Abraham Colfe. He was born in Canterbury in or before 1560. His father and mother, who were zealous Protestants, had a considerable estate at Guisnes, which they lost on the reconquest of Calais by the French in 1558.
Isaac was entered as a commoner of Broadgates Hall, Oxford, in 1576, and proceeded Bachelor of Arts on 17 February 1580, and M. A. on 4 July 1582. Having taken orders he was presented to the vicarage of Stone, Kent on 25 February
1585, and resigned it in 1587, on his appointment to the vicarage of Brookland in the same county.
On 18 June 1596 he was inducted master of Kingsbridge Hospital, Canterbury. He died on 15 June 1597, and was buried in the chapter-house of the cathedral.
He had two sons: Isaac of Christ Church, and Jacob of All Souls" College, Oxford.
