In 1982, she received a degree of Bachelor of Medicine and Bachelor of Surgery from Armed Forces Medical College, Pune, India. In 1991 she graduated from Bombay University with a Doctor of Medicine degree in Pediatrics.
From 1982-2005 Madhuri Kanitkar served a Lieutenant colonel at Indian military. From 1982-1987 she was a Medical officer at Army Medical College Hospital, Jodhpur, India and also in Mumbai, India, 1987-1991. Since 2001 she is an Associate professor at Armed Forces Medical College and Command Hospital, Pune.
He is a member of Indian Pediatrics Nephrology Group, Indian Nephrology Society and Indian Academy of Pediatrics.
He is married to Rajeev Vasant Kanitkar on November 16, 1982 and has 2 children.