Background
He was the eldest son of John V, Prince of Anhalt-Zerbst, by his wife Margaret, daughter of Joachim I Nestor, Elector of Brandenburg.
He was the eldest son of John V, Prince of Anhalt-Zerbst, by his wife Margaret, daughter of Joachim I Nestor, Elector of Brandenburg.
In Zerbst on 16 May 1557 Karl married Anna (5 February 1531 – 13 October 1592), daughter of Barnim XI, Duke of Pomerania. The union was childless.