In 1907, with Giovanni Buitoni, she created the small company Perugina with headquarters in historical center of Perugia and only 15 employees. After the war, the Perugina factory grew to more than one hundred employees. In 1923, a brand of Italian chocolate called Bacio (Kiss) was created.
After the end of the war Luisa created a new company: breeding of poultry and angora rabbits.
1928 - Luisa Spagnoli was the first person to introduce the angora yarn for knitwear with the trademark l"- shawls, boleros and fashionable garments. In the Fair of Milano this innovation was noted and the activity of company expand.
She was diagnosed with cancer. Giovanni Buitoni moved to Paris to ensure the best care and remained with her until her death in Paris in 1935.
He was credited with the invention, patented in 1942, of two objects: a comb for collection of wool and a clamp for tattooing angora rabbits.
Mario built, in 1947, the new factory of the "City dell"angora", around which grew a community, where the party was welfare and recreational cycle phase. In the 1960s, he also founded the playground of the Città della Domenica it:Città della Domenica originally called Spagnolia and even today a destination for visitors.