Levin received a Bachelor of Science degree from the University of Liverpool in 1956. Four years later he earned his Master of Science degree in Physiology at the same university. In 1964, Roy was given a Doctor of Philosophy degree in Physiology from the University of Sheffield.
Levin began his career as a reader in physiology of Biomedical Science Department at the University of Sheffield in 1959 and held it for fourty one year. Also in 1962 he was appointed an assistant lecturer at that university. A year later Roy took a position of a lecturer and then in 1969, he held the position of a senior lecturer at the University of Sheffield.
He worked an assistant professor at the University of Iowa from 1964 to 1965. In 2000, Levin became an honorary research associate of sexual physiology laboratory at Porterbrook Clinic at Sheffield Teaching Hospitals NHS Foundation Trust, where he worked for fifteen years.
Levin was a member of Physiological Society, International Academy of Sex Research and British Gastroenterology Society.
Roy Levin married Margaret Ensor in 1964. They have 2 children.