Background
Doolittle, Russell Francis was born on January 10, 1931 in New Haven. Son of Russell A. and Mary Catherine (Bohan) Doolittle.
(In these days of facile cloning and rapid DNA sequencing,...)
In these days of facile cloning and rapid DNA sequencing, it is not uncommon for investigators to find themselves with a DNA sequence that may or may not code for a known gene product. The sequence is 'open' when read in an appropriate frame, which is to say that there is a long run of amino acid codons before the appearance of a terminator codon. How can we find out if this 'unidentified reading frame' (URF) really codes for a genuine protein, and how can we identify it if it exists? There are two general strategies, both of which can also be applied to the characterization of any 'open reading frame' (ORF), whether or not it has been 'identified'. The first and simplest approach involves computer searching and analysis; the second employs antibodies raised against synthetic peptides patterned on the sequence of the expected gene product. Both methods have been used with great success by many investigators. Each has, nonetheless, its pitfalls and frustrations. This primer is meant to guide the researcher past those obstacles as much as possible. Graduate students and researchers interested in amino acid sequencing; molecular biologists, biochemists, chemists, and biotechnologists.
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Doolittle, Russell Francis was born on January 10, 1931 in New Haven. Son of Russell A. and Mary Catherine (Bohan) Doolittle.
Bachelor, Wesleyan University, 1952. Master of Arts, Trinity College, 1957. Doctor of Philosophy, Harvard University, 1962.
Instructor biochemistry, Amherst (Massachusetts) College, 1961-1962; assistant research biologist, University of California-San Diego, La Jolla, 1964-1965; assistant professor biochemistry, University of California-San Diego, La Jolla, 1965-1967; associate professor, University of California-San Diego, La Jolla, 1967-1972; professor, University of California-San Diego, La Jolla, since 1972; department chairman chemistry, University of California-San Diego, La Jolla, 1981-1984; research professor biology and chemistry, University of California-San Diego, La Jolla, since 1994. Advisor Canada Institute for Advanced Research.
(In these days of facile cloning and rapid DNA sequencing,...)
(Will be shipped from US. Used books may not include compa...)
Served as sergeant United States Army, 1952-1954. Fellow American Association for the Advancement of Science. Member National Academy of Sciences (John Jay Carty award for the Advancement of Science, 2006), American Society Biological Chemistry, American Academy Arts and Sciences, American Philosophical Society (Paul Ehrlich prize 1989, Stein and Moore award 1991).
Married Frances Ann Tynan, June 6, 1931. Children: Lawrence Russell, William Edward.