San-ei Ozeki studied Dutch science in Edo. Then he studied under Seibolt in Nagasaki.
After studying Dutch science in Edo was appointed teacher at a medical school in Sendai and then resigned to study under Seibolt in Nagasaki. Together with Choei Takano, he studied medicine, astronomy and geography, and began translating Dutch books into Japanese. Later was appointed physician to Lord of Kishiwada (Osaka Prefecture).