Soon after graduation from the university Takikawa joined the Ministry of Justice. Leaving government employment he became an assistant professor of his alma mater, and was sent abroad for further studies, and upon return Yokitoki was made a full-fledged professor.
In 1933 his book "Reader of Penal Law" created a sensation when it was banned by the government. Heated protests were made and this incident is still remembered as the famous Takikawa Incident. It happened in October 1932 when Kyoto Imperial University Faculty of Law Professor Takigawa Yukitoki lectured on the need for the judiciary to understand the social roots of deviance when considering individuals who are before them. The climactic moment occurred in May 1933, when Education Minister Hatoyama Ichiro announced that Dr. Takigawa's theory of criminal law advocated Marxist philosophies and suspended him from teaching. The remaining members of the Faculty of Law resigned from their positions in protest, students boycotted classes, and communist sympathisers organised a protest movement. The Ministry of Education suppressed the movement by firing Takigawa.